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Addgene

3xFLAG-CUL2-pCMV7.1
(Plasmid #155020)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 155020 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV7.1
  • Backbone manufacturer
    Sigma Aldrich
  • Backbone size w/o insert (bp) 4717
  • Total vector size (bp) 8889
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cullin 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4172
  • GenBank ID
    NM_001198778.1
  • Entrez Gene
    CUL2
  • Promoter pCMV7.1
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (destroyed during cloning)
  • 3′ cloning site XbaI (destroyed during cloning)
  • 5′ sequencing primer AATGTCGTAATAACCCCGCCCCGTTGACGC
  • 3′ sequencing primer CCTAGTCAGACAAAATGATGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3xFLAG-CUL2-pCMV7.1 was a gift from Andre Catic (Addgene plasmid # 155020 ; http://n2t.net/addgene:155020 ; RRID:Addgene_155020)

  • For your References section:

    The ubiquitin ligase Cullin-1 associates with chromatin and regulates transcription of specific c-MYC target genes. Sweeney MA, Iakova P, Maneix L, Shih FY, Cho HE, Sahin E, Catic A. Sci Rep. 2020 Aug 18;10(1):13942. doi: 10.1038/s41598-020-70610-0. 10.1038/s41598-020-70610-0 PubMed 32811853
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