pDragon-p21
(Plasmid
#155016)
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PurposeFor Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to the cell cycle suppressor p21 in the presence of Cre and (r)tTA activity
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 155016 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAR52
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Vector typeMouse Targeting
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Selectable markersSplit Hygro
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCdkn1a
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Alt namep21
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SpeciesM. musculus (mouse)
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Entrez GeneCdkn1a (a.k.a. CAP20, CDKI, CIP1, Cdkn1, P21, SDI1, Waf1, mda6, p21Cip1, p21WAF)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Mlu (not destroyed)
- 3′ cloning site SnaBI (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDragon-p21 was a gift from Alberto Rosello-Diez (Addgene plasmid # 155016 ; http://n2t.net/addgene:155016 ; RRID:Addgene_155016) -
For your References section:
A collection of genetic mouse lines and related tools for inducible and reversible intersectional mis-expression. Ahmadzadeh E, Bayin NS, Qu X, Singh A, Madisen L, Stephen D, Zeng H, Joyner AL, Rosello-Diez A. Development. 2020 May 28;147(10). pii: dev.186650. doi: 10.1242/dev.186650. 10.1242/dev.186650 PubMed 32366677