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Addgene

pDragon-p21
(Plasmid #155016)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 155016 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    AR52
  • Vector type
    Mouse Targeting
  • Selectable markers
    Split Hygro

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cdkn1a
  • Alt name
    p21
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Cdkn1a (a.k.a. CAP20, CDKI, CIP1, Cdkn1, P21, SDI1, Waf1, mda6, p21Cip1, p21WAF)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Mlu (not destroyed)
  • 3′ cloning site SnaBI (not destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDragon-p21 was a gift from Alberto Rosello-Diez (Addgene plasmid # 155016 ; http://n2t.net/addgene:155016 ; RRID:Addgene_155016)
  • For your References section:

    A collection of genetic mouse lines and related tools for inducible and reversible intersectional mis-expression. Ahmadzadeh E, Bayin NS, Qu X, Singh A, Madisen L, Stephen D, Zeng H, Joyner AL, Rosello-Diez A. Development. 2020 May 28;147(10). pii: dev.186650. doi: 10.1242/dev.186650. 10.1242/dev.186650 PubMed 32366677