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PurposeExpresses the codon-optimized, engineered iSpyMac Cas9 nuclease in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 155014 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneAdapted from pCMV_AncBE4max (#112094)
- Backbone size w/o insert (bp) 6031
- Total vector size (bp) 10111
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameiSpyMac
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SpeciesStreptococcus macacae NCTC 11558
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Insert Size (bp)4080
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MutationPID grafted onto SpyCas9 N-terminal domain, R221K, N394K
- Promoter EF1ɑ
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer EF-1a Forward
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
iSpyMac was a gift from Joseph Jacobson (Addgene plasmid # 155014 ; http://n2t.net/addgene:155014 ; RRID:Addgene_155014) -
For your References section:
A Cas9 with PAM recognition for adenine dinucleotides. Chatterjee P, Lee J, Nip L, Koseki SRT, Tysinger E, Sontheimer EJ, Jacobson JM, Jakimo N. Nat Commun. 2020 May 18;11(1):2474. doi: 10.1038/s41467-020-16117-8. 10.1038/s41467-020-16117-8 PubMed 32424114
Map uploaded by the depositor.
