Sc++
(Plasmid #155011)

• Purpose: Expresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cells
• Depositing Lab: Joseph Jacobson
• Publication: Chatterjee et al Nat Biotechnol. 2020 May 11. pii: 10.1038/s41587-020-0517-0. doi: 10.1038/s41587-020-0517-0.

Backbone

• Vector backbone: Adapted from pCMV_AncBE4max (#112094)
• Backbone size w/o insert (bp): 6050
• Total vector size (bp): 10175
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sc++
• Alt name: Engineered ScCas9 Nuclease
• Species: Streptococcus canis FSL Z3-227
• Insert Size (bp): 4125
• Mutation: T1227K, Loop Substitution from S. anginosus
• Promoter: EF1ɑ

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: EF-1a Forward
• 3′ sequencing primer: EGFP-N

Resource Information

• Supplemental Documents:
  • scpp.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry