pWPI-IRES-Puro-Ak-ACE2
(Plasmid #154985)

• Purpose: Lentiviral vector expressing Human ACE2
• Depositing Lab: Sonja Best
• Publication: Best Lab Plasmids (unpublished)

Backbone

• Vector backbone: pWPI-IRES-Puro-Ak
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ACE2
• Species: H. sapiens (human)
• Insert Size (bp): 2418
• Entrez Gene: ACE2 (a.k.a. ACEH)

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pWPI-IRES-Puro-AK-ACE2.gb
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Information for Lentiviral Prep (Catalog # 154985-LV)

Purpose

Ready-to-use Lentiviral Prep particles produced from pWPI-IRES-Puro-Ak-ACE2 (#154985). In addition to the viral particles, you will also receive purified pWPI-IRES-Puro-Ak-ACE2 plasmid DNA.

Lentiviral particles carrying human ACE2.

Delivery

• Volume: 1mL
• Titer: ≥ 1x10⁶ TU/mL
• Pricing: $150 USD for preparation of 1mL virus + $30 USD for plasmid.
• Storage: Store at -80℃. Thaw just before use and keep on ice.
• Shipment: Viral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

• Packaging Plasmids: psPAX2 (plasmid #12260)
• Envelope: pMD2.G (plasmid #12259)
• Buffer: OptiPro

Biosafety

Requestor is responsible for compliance with their institution's biosafety regulations. Lentivirus is generally considered BSL-2. AAV is generally considered BSL-1, but may require BSL-2 handling depending on the insert. Biosafety Guide

Terms and Licenses

• Academic/Nonprofit Terms:
  • Terms of Use for Viral Vectors
• Industry Terms:
  • Not Available to Industry

Viral Quality Control

Titering Method:

• ddPCR assay: 293T cells were transduced with serial dilutions of 154985-LV, harvested several days later, and genomic DNA was isolated. Copies of RRE were measured and normalized to RPP30.

Notes:

• PCR confirmation of insert: PCRs were carried out on the viral preparation with primers targeting the backbone and ACE2 sequences. The PCR products were visualized on an agarose gel for size confirmation.

Visit our viral production page for more information.