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Addgene

pCSDest-HA-TMPRSS2
(Plasmid #154963)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 154963 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pCSDest
  • Modifications to backbone
    Added N-terminal HA tag, Tag added by Q5-site directed mutagenesis
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HA-TMPRSS2
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_005656.4
  • Entrez Gene
    TMPRSS2 (a.k.a. PRSS10)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    https://www.addgene.org/53887/
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSDest-HA-TMPRSS2 was a gift from Utpal Pajvani (Addgene plasmid # 154963 ; http://n2t.net/addgene:154963 ; RRID:Addgene_154963)
  • For your References section:

    Angiotensin converting enzyme 2 is a novel target of the gamma-secretase complex. Bartolome A, Liang J, Wang P, Ho DD, Pajvani UB. Sci Rep. 2021 May 7;11(1):9803. doi: 10.1038/s41598-021-89379-x. 10.1038/s41598-021-89379-x PubMed 33963249