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Purposeencodes transcriptional reporter of SMAD2/3
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 154961 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePB510B
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Backbone manufacturerSystem Biosciences
- Backbone size w/o insert (bp) 5700
- Total vector size (bp) 7064
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Vector typeMammalian Expression ; Piggybac
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemScarlet
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SpeciesSynthetic
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Tag
/ Fusion Protein
- NLS-PEST
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB_SBE4:mScarlet-I-NLS was a gift from Ewa Bielczyk-Maczynska & Joshua Knowles (Addgene plasmid # 154961 ; http://n2t.net/addgene:154961 ; RRID:Addgene_154961) -
For your References section:
TGF-beta is insufficient to induce adipocyte state loss without concurrent PPARgamma downregulation. Taylor B, Shah A, Bielczyk-Maczynska E. Sci Rep. 2020 Aug 21;10(1):14084. doi: 10.1038/s41598-020-71100-z. 10.1038/s41598-020-71100-z PubMed 32826933