pCS2-hDKK3-flag
(Plasmid #15496)

• Depositing Lab: Sergei Sokol
• Publication: Krupnik et al Gene. 1999 Oct 1. 238(2):301-13.

Backbone

• Vector backbone: pCS2
• Backbone size w/o insert (bp): 4100
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DKK3
• Alt name: Dickkopf-3
• Species: H. sapiens (human)
• Insert Size (bp): 1130
• Mutation: See note below
• GenBank ID: AF177396
• Entrez Gene: DKK3 (a.k.a. CRRL, REIC, RIG)
• Tag / Fusion Protein:
  • flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See author's map for details.

Addgene NGS identified G347R variant compared to NCBI reference [AAF02676.1].