pET28-Nsp14-TEV-Strep
(Plasmid #154953)

• Purpose: Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable Strep tag in E. coli
• Depositing Lab: Justin Kollman
• Publication: Plasmids for the Expression and Co-Expression of COVID-19 Proteins Nsp10 and Nsp14 (unpublished)

Backbone

• Vector backbone: pET28
• Backbone size w/o insert (bp): 5233
• Total vector size (bp): 6865
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nsp14-TEV-Strep
• Alt name: Non-structural protein 14
• Species: SARS-CoV-2
• Insert Size (bp): 1632
• GenBank ID: MN985325
• Entrez Gene: ORF1ab (a.k.a. GU280_gp01)
• Promoter: T7 Promoter
• Tag / Fusion Protein:
  • TEV-Strep (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 Promoter
• 3′ sequencing primer: T7 Terminator

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gene was PCR amplified from the relevant plasmid described in Gordon et al, Nature (2020); doi: 10.1038/s41586-020-2286-9

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA