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Addgene

pCS2-hDKK2-flag
(Plasmid #15495)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15495 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DKK2
  • Alt name
    Dickkopf-2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    812
  • GenBank ID
    AF177395
  • Entrez Gene
    DKK2 (a.k.a. DKK-2)
  • Tag / Fusion Protein
    • flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map for details.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-hDKK2-flag was a gift from Sergei Sokol (Addgene plasmid # 15495 ; http://n2t.net/addgene:15495 ; RRID:Addgene_15495)

  • For your References section:

    Functional and structural diversity of the human Dickkopf gene family. Krupnik VE, Sharp JD, Jiang C, Robison K, Chickering TW, Amaravadi L, Brown DE, Guyot D, Mays G, Leiby K, Chang B, Duong T, Goodearl AD, Gearing DP, Sokol SY, McCarthy SA. Gene. 1999 Oct 1. 238(2):301-13. 10.1016/S0378-1119(99)00365-0 PubMed 10570958
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