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Addgene

dCas13d-dsRBD-APEX2
(Plasmid #154939)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154939 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PB-TetON
  • Backbone manufacturer
    George Church lab
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP
  • Promoter TRE3G
  • Tag / Fusion Protein
    • V5

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There are ITRs for piggybac transposase insertion but no transposase is in the plasmid itself.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    dCas13d-dsRBD-APEX2 was a gift from Alice Ting (Addgene plasmid # 154939 ; http://n2t.net/addgene:154939 ; RRID:Addgene_154939)

  • For your References section:

    RNA-protein interaction mapping via MS2- or Cas13-based APEX targeting. Han S, Zhao BS, Myers SA, Carr SA, He C, Ting AY. Proc Natl Acad Sci U S A. 2020 Aug 24. pii: 2006617117. doi: 10.1073/pnas.2006617117. 10.1073/pnas.2006617117 PubMed 32839320
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