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PurposeNLS-V5-MCP-APEX2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 154936 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLX304
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNLS-V5-MCP-APEX2
- Promoter CMV
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Tag
/ Fusion Protein
- V5
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV
- 3′ sequencing primer WPRE (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MCP-APEX2 was a gift from Alice Ting (Addgene plasmid # 154936 ; http://n2t.net/addgene:154936 ; RRID:Addgene_154936) -
For your References section:
RNA-protein interaction mapping via MS2- or Cas13-based APEX targeting. Han S, Zhao BS, Myers SA, Carr SA, He C, Ting AY. Proc Natl Acad Sci U S A. 2020 Aug 24. pii: 2006617117. doi: 10.1073/pnas.2006617117. 10.1073/pnas.2006617117 PubMed 32839320