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Addgene

pMXS-IRES-BLAST PHGDH
(Plasmid #154917)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154917 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMXS-IRES-BLAST
  • Backbone size w/o insert (bp) 5600
  • Total vector size (bp) 7200
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PHGDH
  • Alt name
    3PGDH, 3 phosphoglycerate dehydrogenase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • GenBank ID
    NM_006623.4
  • Entrez Gene
    PHGDH (a.k.a. 3-PGDH, 3PGDH, HEL-S-113, NLS, NLS1, PDG, PGAD, PGD, PGDH, PHGDHD, SERA)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer AGTAGACGGCATCGCAGCTTGGATA
  • 3′ sequencing primer CGGCCTTATTCCAAGCGGCTTCG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMXS-IRES-BLAST PHGDH was a gift from Richard Possemato (Addgene plasmid # 154917 ; http://n2t.net/addgene:154917 ; RRID:Addgene_154917)

  • For your References section:

    Richard Possemato Lab plasmids. Richard Possemato. Richard Possemato Lab plasmids PubMed 32446203
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