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Addgene

pCW57.1 hPHGDH
(Plasmid #154916)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154916 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCW57.1
  • Backbone size w/o insert (bp) 15200
  • Total vector size (bp) 16800
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PHGDH
  • Alt name
    3PGDH, Phosphoglycerate dehydrogenase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • GenBank ID
    NM_006623.4
  • Entrez Gene
    PHGDH (a.k.a. 3-PGDH, 3PGDH, HEL-S-113, NLS, NLS1, PDG, PGAD, PGD, PGDH, PHGDHD, SERA)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer AGCTCGTTTAGTGAACCGTCAGATC
  • 3′ sequencing primer GAACGGACGTGAAGAATGTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCW57.1 hPHGDH was a gift from Richard Possemato (Addgene plasmid # 154916 ; http://n2t.net/addgene:154916 ; RRID:Addgene_154916)

  • For your References section:

    Richard Possemato Lab plasmids. Richard Possemato. Richard Possemato Lab plasmids PubMed 32446203
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