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Addgene

GFP-MuTMC2truncated
(Plasmid #154909)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154909 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac-1
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP-MuTMC2truncated
  • Species
    Synthetic
  • Mutation
    Truncations of the residues 1-179 and 691-716
  • Tag / Fusion Protein
    • 8histidine-EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site HindII (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-MuTMC2truncated was a gift from Motoyuki Hattori (Addgene plasmid # 154909 ; http://n2t.net/addgene:154909 ; RRID:Addgene_154909)

  • For your References section:

    TMC1 and TMC2 Proteins Are Pore-Forming Subunits of Mechanosensitive Ion Channels. Jia Y, Zhao Y, Kusakizako T, Wang Y, Pan C, Zhang Y, Nureki O, Hattori M, Yan Z. Neuron. 2020 Jan 22;105(2):310-321.e3. doi: 10.1016/j.neuron.2019.10.017. Epub 2019 Nov 21. 10.1016/j.neuron.2019.10.017 PubMed 31761710
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