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Addgene

Myc-Xdsh-pCS2 (RXF)
(Plasmid #15490)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15490 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Backbone size w/o insert (bp) 4300
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dishevelled
  • Alt name
    Xdsh
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    3300
  • GenBank ID
    U31552
  • Entrez Gene
    dvl2.L (a.k.a. XELAEV_18019661mg, dishevelled, dsh, dvl, dvl2, xdd1, xdsh)
  • Tag / Fusion Protein
    • myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map for details. 1 kb EcoRI-ApaI plus 2.3 kb ApaI-SalI fragments of Xdsh cDNA were cloned into 4.3 kb EcoRI-XhoI cut pCS2-myc vector in-frame.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Myc-Xdsh-pCS2 (RXF) was a gift from Sergei Sokol (Addgene plasmid # 15490 ; http://n2t.net/addgene:15490 ; RRID:Addgene_15490)

  • For your References section:

    Analysis of Dishevelled signalling pathways during Xenopus development. Sokol SY. Curr Biol. 1996 Nov 1. 6(11):1456-67. 10.1016/S0960-9822(96)00750-6 PubMed 8939601
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