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CSIV-TRE-ASCL1
(Plasmid #154883)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154883 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CSIV-TRE
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ASCL1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    711
  • Entrez Gene
    ASCL1 (a.k.a. ASH1, HASH1, MASH1, bHLHa46)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer ttagtgaaccgtcagatcgc
  • 3′ sequencing primer gcaatgccccaaccagtg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CSIV-TRE-ASCL1 was a gift from Hideyuki Okano (Addgene plasmid # 154883 ; http://n2t.net/addgene:154883 ; RRID:Addgene_154883)

  • For your References section:

    Direct Neuronal Reprogramming of Common Marmoset Fibroblasts by ASCL1, microRNA-9/9*, and microRNA-124 Overexpression. Nemoto A, Kobayashi R, Yoshimatsu S, Sato Y, Kondo T, Yoo AS, Shiozawa S, Okano H. Cells. 2020 Dec 22;10(1). pii: cells10010006. doi: 10.3390/cells10010006. 10.3390/cells10010006 PubMed 33375083
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