pPB-tetpA(hBCL2-miR9/9*-124)-iRPT
(Plasmid #154881)

• Purpose: Doxycycline-dependently expresses human BCL2 and murine miR9/9*-124. Ubiquitously expresses iRFP713, Puromycin resistance gene, and rtTAM2.
• Depositing Lab: Hideyuki Okano
• Publication: Nemoto et al Cells. 2020 Dec 22;10(1). pii: cells10010006. doi: 10.3390/cells10010006.

Backbone

• Vector backbone: pPB-tetpA (Addgene #140763)
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: iRFP713
• Species: Synthetic
• Insert Size (bp): 948

Gene/Insert 2

• Gene/Insert name: Puromycin resistance gene
• Species: E.coli
• Insert Size (bp): 597

Gene/Insert 3

• Gene/Insert name: rtTAM2
• Species: Synthetic
• Insert Size (bp): 747

Gene/Insert 4

• Gene/Insert name: BCL2
• Species: H. sapiens (human)
• Insert Size (bp): 717
• Entrez Gene: BCL2 (a.k.a. Bcl-2, PPP1R50)

Gene/Insert 5

• Gene/Insert name: miR9/9*-124
• Species: M. musculus (mouse)
• Insert Size (bp): 656

Resource Information

• Supplemental Documents:
  • pPB-tetpA(hBCL2-miR9 9*-124)-iRPT.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry