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pLVX-Puro-mGSDME F2A
(Plasmid #154878)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154878 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX-Puro
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 8102
  • Total vector size (bp) 9600
  • Modifications to backbone
    none
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GSDME
  • Alt name
    DNFA5
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1539
  • Mutation
    changes Phenylalanine 2 to Alanine
  • GenBank ID
    NM_018769
  • Entrez Gene
    Gsdme (a.k.a. 2310037D07Rik, 4932441K13Rik, Dfna5, Dfna5h, EG14210, Fin15)
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer agcgctaccggactcagatctcgagATGGCTGCCAAAGCAACTCGAAAT
  • 3′ sequencing primer AGGACATGCTACAGGTCAAGACTAGggatcccgcgactctagataattc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mouse GSDME CDS was amplified form cDNA derived from EMT6 cell. It contains several variants in DNA sequence (540 T-C, 600 G-T, 609 G-A, 1011 T-C) but the amino acid is not affected.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-Puro-mGSDME F2A was a gift from Judy Lieberman (Addgene plasmid # 154878 ; http://n2t.net/addgene:154878 ; RRID:Addgene_154878)

  • For your References section:

    Gasdermin E suppresses tumour growth by activating anti-tumour immunity. Zhang Z, Zhang Y, Xia S, Kong Q, Li S, Liu X, Junqueira C, Meza-Sosa KF, Mok TMY, Ansara J, Sengupta S, Yao Y, Wu H, Lieberman J. Nature. 2020 Mar;579(7799):415-420. doi: 10.1038/s41586-020-2071-9. Epub 2020 Mar 11. 10.1038/s41586-020-2071-9 PubMed 32188940
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