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pLVX-Puro-mGSDME
(Plasmid #154877)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154877 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX-Puro
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 8102
  • Total vector size (bp) 9600
  • Modifications to backbone
    none
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GSDME
  • Alt name
    DNFA5
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1539
  • GenBank ID
    NC_000072
  • Entrez Gene
    Gsdme (a.k.a. 2310037D07Rik, 4932441K13Rik, Dfna5, Dfna5h, EG14210, Fin15)
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer agcgctaccggactcagatctcgagATGTTTGCCAAAGCAACTCGAAAT
  • 3′ sequencing primer AGGACATGCTACAGGTCAAGACTAGggatcccgcgactctagataattc
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-Puro-mGSDME was a gift from Judy Lieberman (Addgene plasmid # 154877 ; http://n2t.net/addgene:154877 ; RRID:Addgene_154877)

  • For your References section:

    Gasdermin E suppresses tumour growth by activating anti-tumour immunity. Zhang Z, Zhang Y, Xia S, Kong Q, Li S, Liu X, Junqueira C, Meza-Sosa KF, Mok TMY, Ansara J, Sengupta S, Yao Y, Wu H, Lieberman J. Nature. 2020 Mar;579(7799):415-420. doi: 10.1038/s41586-020-2071-9. Epub 2020 Mar 11. 10.1038/s41586-020-2071-9 PubMed 32188940
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