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Addgene

pAAV-hSyn-fDIO-rM3D(Gs)-mCherry-WPREpA
(Plasmid #154869)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154869 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-hSyn-Flp-WPRE
  • Backbone manufacturer
    Gether lab - modified from Addgene plasmid #55641
  • Backbone size w/o insert (bp) 4824
  • Total vector size (bp) 6792
  • Vector type
    Mammalian Expression, AAV ; FLP-FRT

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rM3D(Gs)-mCherry
  • Alt name
    Gs-DREADD
  • Insert Size (bp)
    1968
  • Promoter Human Synapsin-1
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Asc1 (not destroyed)
  • 3′ cloning site Nhe1 (not destroyed)
  • 5′ sequencing primer actcagcgctgcctcagtct
  • 3′ sequencing primer gatacaaaggcattaaagcagcg
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The insert is derived from Addgene #50458 and the backbone is modified (Ef1a promoter replaced with human synapsin promoter Addgene # Plasmid #55641).

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hSyn-fDIO-rM3D(Gs)-mCherry-WPREpA was a gift from Ulrik Gether (Addgene plasmid # 154869 ; http://n2t.net/addgene:154869 ; RRID:Addgene_154869)
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