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Addgene

pcDNA-HA-PfROM4 (G470A/G471N)
(Plasmid #154859)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154859 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA 3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7900
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PfROM4
  • Alt name
    Rhomboid protease ROM4
  • Species
    Synthetic
  • Insert Size (bp)
    2400
  • Mutation
    Glycine 470 changed to Alanine and Glycine 471 changed to Asparagine
  • GenBank ID
    DQ986370
  • Entrez Gene
    PF3D7_0506900 (a.k.a. PF3D7_0506900)
  • Promoter CMV
  • Tag / Fusion Protein
    • triple HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-HA-PfROM4 (G470A/G471N) was a gift from Sinisa Urban (Addgene plasmid # 154859 ; http://n2t.net/addgene:154859 ; RRID:Addgene_154859)

  • For your References section:

    Designed Parasite-Selective Rhomboid Inhibitors Block Invasion and Clear Blood-Stage Malaria. Gandhi S, Baker RP, Cho S, Stanchev S, Strisovsky K, Urban S. Cell Chem Biol. 2020 Aug 31. pii: S2451-9456(20)30333-0. doi: 10.1016/j.chembiol.2020.08.011. 10.1016/j.chembiol.2020.08.011 PubMed 32888502
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