pMS74 (synthetic guide::∆HYGR::unc-54 3' UTR^SEC)
(Plasmid #154838)

• Purpose: Insertion of split hygromycin landing pad into ChrII:8420157 site C. elegans strains via CRISPR with SEC selection
• Depositing Lab: Patrick Phillips
• Publication: Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.401400. doi: 10.1534/g3.120.401400.

Backbone

• Vector backbone: pMS4
• Backbone size w/o insert (bp): 10683
• Total vector size (bp): 11930
• Vector type: Worm Expression, Cre/Lox, CRISPR
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: synthetic guide::∆HYGR::unc-54 3' UTR
• Species: C. elegans (nematode), Synthetic
• Insert Size (bp): 1225
• Mutation: ∆HYGR is promoterless and encodes aa 60-341

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: gcccaggagaacacgttag
• 3′ sequencing primer: TtGGCGTCGATCATCCTTAG

Resource Information

• Supplemental Documents:
  • pMS74.gb
• A portion of this plasmid was derived from a plasmid made by: Hygromycin resistance gene fragment was modified from pCFJ1663 which was received from Addgene.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Additional protocol information can be found at: github.com/phillips-lab/SLP