hSERT pcDNA3
(Plasmid #15483)

• Depositing Lab: Randy Blakely
• Publication: Ramamoorthy et al Proc Natl Acad Sci U S A. 1993 Mar 15. 90(6):2542-6.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hSERT
• Alt name: serotonin transporter
• Alt name: 5HT transporter
• Alt name: solute carrier family 6 member 4 (SLC6A4)
• Species: H. sapiens (human)
• Insert Size (bp): 2500
• Entrez Gene: SLC6A4 (a.k.a. 5-HTT, 5-HTTLPR, 5HTT, HTT, OCD1, SERT, SERT1, hSERT)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry