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Addgene

pLKO.1-mPRAS40
(Plasmid #15480)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15480 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLKO.1
  • Backbone size w/o insert (bp) 7000
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRAS40 shRNA
  • Alt name
    PRAS40
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    59
  • Entrez Gene
    Akt1s1 (a.k.a. 1110012J22Rik, AI227026, Lobe, Lobel, PRAS40)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer LKO.1 5'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Target sequence for mouse PRAS40 is 5'-GAGCCCACTGAAACAGAGACA-3'.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO.1-mPRAS40 was a gift from Do-Hyung Kim (Addgene plasmid # 15480 ; http://n2t.net/addgene:15480 ; RRID:Addgene_15480)

  • For your References section:

    Insulin signalling to mTOR mediated by the Akt/PKB substrate PRAS40. Vander Haar E, Lee SI, Bandhakavi S, Griffin TJ, Kim DH. Nat Cell Biol. 2007 Mar . 9(3):316-23. 10.1038/ncb1547 PubMed 17277771
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