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Addgene

pRK5-myc-PRAS40 T246A
(Plasmid #15479)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15479 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5-Myc
  • Backbone size w/o insert (bp) 4754
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRAS40
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    800
  • Mutation
    Thr246 to Ala
  • Entrez Gene
    AKT1S1 (a.k.a. Lobe, PRAS40)
  • Tag / Fusion Protein
    • myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5-myc-PRAS40 T246A was a gift from Do-Hyung Kim (Addgene plasmid # 15479 ; http://n2t.net/addgene:15479 ; RRID:Addgene_15479)

  • For your References section:

    Insulin signalling to mTOR mediated by the Akt/PKB substrate PRAS40. Vander Haar E, Lee SI, Bandhakavi S, Griffin TJ, Kim DH. Nat Cell Biol. 2007 Mar . 9(3):316-23. 10.1038/ncb1547 PubMed 17277771
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