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PurposeExpresses synonymized MS2 coat protein dimer (MCP) fused to hyperactive ADAR catalytic domain
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 154787 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonephage-ubc-nls-ha-tdMCP-gfp (Addgene Plasmid #40649)
- Backbone size w/o insert (bp) 6200
- Total vector size (bp) 9169
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Vector typeMammalian Expression, Lentiviral
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Selectable markersGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAdenosine deaminse
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Alt nameADAR
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SpeciesD. melanogaster (fly), Synthetic
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Insert Size (bp)1281
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MutationSynonymized serine to prevent autoediting of ADAR, hyperediting mutation E488Q of ADAR
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Entrez GeneAdar (a.k.a. Dmel_CG12598, ADAR, ADAR1, CG12598, Dmel\CG12598, EG:BACN35H14.1, adar, adr, cg12598, dADAR, dAdar, dadar, hypnos-2)
- Promoter UBC
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Tags
/ Fusion Proteins
- stdMCP (N terminal on insert)
- V5 (C terminal on insert)
- GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (destroyed during cloning)
- 3′ cloning site SphI (not destroyed)
- 5′ sequencing primer UBC_F (5'-CGCCGATGATTATATAAGGA-3')
- 3′ sequencing primer GFP_R (5'-GCATCACCTTCACCTTCACC-3') (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP was a gift from Robert Singer (Addgene plasmid # 154787 ; http://n2t.net/addgene:154787 ; RRID:Addgene_154787) -
For your References section:
MS2-TRIBE Evaluates Both Protein-RNA Interactions and Nuclear Organization of Transcription by RNA Editing. Biswas J, Rahman R, Gupta V, Rosbash M, Singer RH. iScience. 2020 Jun 28;23(7):101318. doi: 10.1016/j.isci.2020.101318. 10.1016/j.isci.2020.101318 PubMed 32674054