pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP
(Plasmid #154786)

• Purpose: Expresses mCherry fused to hyperactive ADAR catalytic domain, control plasmid for HyperTRIBE
• Depositing Lab: Robert Singer
• Publication: Biswas et al iScience. 2020 Jun 28;23(7):101318. doi: 10.1016/j.isci.2020.101318.

Backbone

• Vector backbone: phage-ubc-nls-ha-tdMCP-gfp (Addgene Plasmid #40649)
• Backbone size w/o insert (bp): 6200
• Total vector size (bp): 9169
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Adenosine deaminse
• Alt name: ADAR
• Species: D. melanogaster (fly), Synthetic
• Insert Size (bp): 1281
• Mutation: Synonymized serine to prevent autoediting of ADAR, hyperediting mutation E488Q of ADAR
• Entrez Gene: Adar (a.k.a. Dmel_CG12598, ADAR, ADAR1, CG12598, Dmel\CG12598, EG:BACN35H14.1, adar, adr, cg12598, dADAR, dAdar, dadar, hypnos-2)
• Promoter: UBC
• Tags / Fusion Proteins:
  • mCherry (N terminal on insert)
  • V5 (C terminal on insert)
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (destroyed during cloning)
• 3′ cloning site: SphI (not destroyed)
• 5′ sequencing primer: UBC_F (5'-CGCCGATGATTATATAAGGA-3')
• 3′ sequencing primer: GFP_R (5'-GCATCACCTTCACCTTCACC-3')

Resource Information

• Supplemental Documents:
  • PHAGE_UBC_mCherry_serinemod_E488QADAR_p2A_yGFP
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry