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pUC_h7SK M15 (UUA)
(Plasmid #154785)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154785 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC
  • Backbone manufacturer
    IDT
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mma PylT
  • Species
    Methanosarcina mazei
  • Insert Size (bp)
    312
  • Mutation
    UUA anticodon and G10A, G14U, G16U, C20U, C52A
  • Promoter h7SK

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

M15 PylT described by Serfling et al., 2018 (PMID: 29177436 )

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC_h7SK M15 (UUA) was a gift from Simon Elsaesser (Addgene plasmid # 154785 ; http://n2t.net/addgene:154785 ; RRID:Addgene_154785)

  • For your References section:

    Site-Specific Incorporation of Two ncAAs for Two-Color Bioorthogonal Labeling and Crosslinking of Proteins on Live Mammalian Cells. Meineke B, Heimgartner J, Eirich J, Landreh M, Elsasser SJ. Cell Rep. 2020 Jun 23;31(12):107811. doi: 10.1016/j.celrep.2020.107811. 10.1016/j.celrep.2020.107811 PubMed 32579937
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