pAS_4xhyb PylT A41AA C55A CRFR1 95TAG 263TAA-HA
(Plasmid #154781)

• Purpose: plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and rat CRFR1 95TAG 263TAA, for transient transfection or stable, piggybac-mediated, integration
• Depositing Lab: Simon Elsaesser
• Publication: Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi: 10.1016/j.celrep.2020.107811.

Backbone

• Vector backbone: pAS
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CRFR1
• Species: R. norvegicus (rat)
• Insert Size (bp): 1245
• Mutation: 95TAG 263TAA in CRFR1-HA, hybrid PylT with A41AA and C55A mutation
• Entrez Gene: Crhr1 (a.k.a. CRF-R, CRF-R-1, CRF-R1, CRF1, CRFR-1, CRFR1, CRH-R, CRH-R 1, CRH-R-1, CRH-R1)
• Promoter: EF1
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

dual suppression of rat CRFR1 described by Serfling et al., 2018 (PMID: 29177436 )