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pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAA 442TAG
(Plasmid #154778)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154778 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAS
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LaG17-SynNotch
  • Species
    Synthetic
  • Insert Size (bp)
    1476
  • Mutation
    204TAA 442TAG in LaG17-SynNotch, hybrid PylT with A41AA and C55A mutation
  • Promoter EF1
  • Tag / Fusion Protein
    • LaG17 (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SynNotch described by Morsut et al. 2016 (PMID: 26830878)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAA 442TAG was a gift from Simon Elsaesser (Addgene plasmid # 154778 ; http://n2t.net/addgene:154778 ; RRID:Addgene_154778)

  • For your References section:

    Site-Specific Incorporation of Two ncAAs for Two-Color Bioorthogonal Labeling and Crosslinking of Proteins on Live Mammalian Cells. Meineke B, Heimgartner J, Eirich J, Landreh M, Elsasser SJ. Cell Rep. 2020 Jun 23;31(12):107811. doi: 10.1016/j.celrep.2020.107811. 10.1016/j.celrep.2020.107811 PubMed 32579937
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