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pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRS
(Plasmid #154774)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154774 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAS
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mma PylRS
  • Species
    Methanosarcina mazei
  • Insert Size (bp)
    2619
  • Mutation
    in PylT: UUA anticodon and G10A, G14U, G16U, C20U, C52A
  • Entrez Gene
    pylS (a.k.a. MSMAC_RS17105, MSMAC_3241)
  • Promoter EF1
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

M15 PylT described by Serfling et al., 2018 (PMID: 29177436 )

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRS was a gift from Simon Elsaesser (Addgene plasmid # 154774 ; http://n2t.net/addgene:154774 ; RRID:Addgene_154774)

  • For your References section:

    Site-Specific Incorporation of Two ncAAs for Two-Color Bioorthogonal Labeling and Crosslinking of Proteins on Live Mammalian Cells. Meineke B, Heimgartner J, Eirich J, Landreh M, Elsasser SJ. Cell Rep. 2020 Jun 23;31(12):107811. doi: 10.1016/j.celrep.2020.107811. 10.1016/j.celrep.2020.107811 PubMed 32579937
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