pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125A
(Plasmid #154773)

• Purpose: plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration
• Depositing Lab: Simon Elsaesser
• Publication: Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi: 10.1016/j.celrep.2020.107811.

Backbone

• Vector backbone: pAS
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: G1 PylRS
• Alt name: 1452364
• Species: methanogenic archaeon mixed culture ISO4-G1
• Insert Size (bp): 846
• Mutation: Y125A in G1PylS, hybrid PylT with A41AA and C55A mutation
• Entrez Gene: MJ_RS07805 (a.k.a. MJ_RS07805, MJ1460, MJ_1460)
• Promoter: EF1
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry