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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15477 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLKO.1
- Backbone size w/o insert (bp) 7000
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Vector typeMammalian Expression, Lentiviral, RNAi
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePRAS40 shRNA
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Alt namePRAS40
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SpeciesH. sapiens (human)
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Insert Size (bp)59
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Entrez GeneAKT1S1 (a.k.a. Lobe, PRAS40)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer LKO.1 5' (Common Sequencing Primers)
Resource Information
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Reference
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Target sequence for human PRAS40 is 5'-CAGCTGGGCATTAGTGATAAT-3'.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLKO.1-PRAS40 was a gift from Do-Hyung Kim (Addgene plasmid # 15477 ; http://n2t.net/addgene:15477 ; RRID:Addgene_15477) -
For your References section:
Insulin signalling to mTOR mediated by the Akt/PKB substrate PRAS40. Vander Haar E, Lee SI, Bandhakavi S, Griffin TJ, Kim DH. Nat Cell Biol. 2007 Mar . 9(3):316-23. 10.1038/ncb1547 PubMed 17277771