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pAS_4xG1 PylT FLAG-G1 PylRS Y125A
(Plasmid #154769)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154769 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAS
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    G1 PylRS
  • Alt name
    1452364
  • Species
    methanogenic archaeon mixed culture ISO4-G1
  • Insert Size (bp)
    846
  • Mutation
    Y125A
  • Entrez Gene
    MJ_RS07805 (a.k.a. MJ_RS07805, MJ1460, MJ_1460)
  • Promoter EF1
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAS_4xG1 PylT FLAG-G1 PylRS Y125A was a gift from Simon Elsaesser (Addgene plasmid # 154769 ; http://n2t.net/addgene:154769 ; RRID:Addgene_154769)

  • For your References section:

    Site-Specific Incorporation of Two ncAAs for Two-Color Bioorthogonal Labeling and Crosslinking of Proteins on Live Mammalian Cells. Meineke B, Heimgartner J, Eirich J, Landreh M, Elsasser SJ. Cell Rep. 2020 Jun 23;31(12):107811. doi: 10.1016/j.celrep.2020.107811. 10.1016/j.celrep.2020.107811 PubMed 32579937
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