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Addgene

pAS_FLAG-Mma PylRS
(Plasmid #154762)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154762 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAS
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mma PylRS
  • Species
    Methanosarcina mazei
  • Insert Size (bp)
    2619
  • Entrez Gene
    pylS (a.k.a. MSMAC_RS17105, MSMAC_3241)
  • Promoter EF1
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' Cloning Site: NheI; 3' Cloning Site: SalI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAS_FLAG-Mma PylRS was a gift from Simon Elsaesser (Addgene plasmid # 154762 ; http://n2t.net/addgene:154762 ; RRID:Addgene_154762)

  • For your References section:

    Methanomethylophilus alvus Mx1201 provides basis for mutual orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in mammalian cells. Meineke B, Heimgartner J, Lafranchi L, Elsasser SJ. ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571. 10.1021/acschembio.8b00571 PubMed 30260624
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