pAT03-Apr
(Plasmid
#154756)
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PurposeFor curing of the resistance marker of RecET recombinase system in Acinetobacter baumannii, Aprar
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 154756 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAT03
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Apramycin, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFLP recombinase
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAT03-Apr was a gift from Sheng Yang (Addgene plasmid # 154756 ; http://n2t.net/addgene:154756 ; RRID:Addgene_154756) -
For your References section:
New Mutations Involved in Colistin Resistance in Acinetobacter baumannii. Sun B, Liu H, Jiang Y, Shao L, Yang S, Chen D. mSphere. 2020 Apr 1;5(2). pii: 5/2/e00895-19. doi: 10.1128/mSphere.00895-19. 10.1128/mSphere.00895-19 PubMed 32238571