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SARS-CoV-2 S HexaPro
(Plasmid #154754)

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Item Catalog # Description Quantity Price (USD)
Plasmid 154754 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pαH
  • Backbone size w/o insert (bp) 4746
  • Total vector size (bp) 8370
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Spike (HexaPro variant)
  • Species
    Severe acute respiratory syndrome coronavirus 2
  • Insert Size (bp)
    3624
  • Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site) replaced with 'GSAS'; F817P, A892P, A899P, A942P, K986P, V987P
  • GenBank ID
    MN908947
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Tags / Fusion Proteins
    • HRV 3C cleavage site (before tags) (C terminal on backbone)
    • 8X His tag (C terminal on backbone)
    • 2X Strep-Tag II (C terminal on backbone)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SARS-CoV-2 S HexaPro was a gift from Jason McLellan (Addgene plasmid # 154754 ; http://n2t.net/addgene:154754 ; RRID:Addgene_154754)

  • For your References section:

    Structure-based design of prefusion-stabilized SARS-CoV-2 spikes. Hsieh CL, Goldsmith JA, Schaub JM, DiVenere AM, Kuo HC, Javanmardi K, Le KC, Wrapp D, Lee AG, Liu Y, Chou CW, Byrne PO, Hjorth CK, Johnson NV, Ludes-Meyers J, Nguyen AW, Park J, Wang N, Amengor D, Lavinder JJ, Ippolito GC, Maynard JA, Finkelstein IJ, McLellan JS. Science. 2020 Sep 18;369(6510):1501-1505. doi: 10.1126/science.abd0826. Epub 2020 Jul 23. 10.1126/science.abd0826 PubMed 32703906
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