pK-myc-Par6B
(Plasmid #15473)

• Depositing Lab: Ian Macara
• Publication: Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

Backbone

• Vector backbone: pKmyc
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Partitioning-defective protein 6B
• Alt name: Par6B
• Alt name: PAR-6B
• Species: M. musculus (mouse)
• Insert Size (bp): 1115
• GenBank ID: AF252291
• Entrez Gene: Pard6b (a.k.a. Par6b)
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: CAGGTCCAACTGCACCTCGGTTC
• 3′ sequencing primer: TTTGTGATGCTATTGCTTTATTTG TA

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry