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Addgene

pEPKKα2KN0100
(Plasmid #154621)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154621 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDGB3_?2
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MinSyn_067 Nanoluc expression cassette [MinSyn_067::LucN::Flag::g7T]
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEPKKα2KN0100 was a gift from Nicola Patron (Addgene plasmid # 154621 ; http://n2t.net/addgene:154621 ; RRID:Addgene_154621)

  • For your References section:

    Rational design of minimal synthetic promoters for plants. Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ. Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. 10.1093/nar/gkaa682 PubMed 32856047
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