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pGEM-MNT-1
(Plasmid #15462)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15462 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM4
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2700
  • Vector type
    cloning and transcription vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    neurotensin
  • Alt name
    neurotensin exon4 + 3' UTR
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    805
  • Entrez Gene
    Nts (a.k.a. 5033428E16Rik, NMN-125, NN, NT, NT/N, NTS1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NsiI (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Paul R. Dobner University of Massachusetts Department of Molecular Genetics and Microbiology

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is a stretch of about 800bp encompassing the 4th exon of the neurotensin gene in mouse. It can be used as an in situ probe.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM-MNT-1 was a gift from Peter Mombaerts (Addgene plasmid # 15462 ; http://n2t.net/addgene:15462 ; RRID:Addgene_15462)

  • For your References section:

    Development and topography of the lateral olfactory tract in the mouse: imaging by genetically encoded and injected fluorescent markers. Walz A, Omura M, Mombaerts P. J Neurobiol. 2006 Jul . 66(8):835-46. 10.1002/neu.20266 PubMed 16673392
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