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Addgene

pBS-NT-AscI
(Plasmid #15461)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15461 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBluescript II SK(-)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2900

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    neurotensin
  • Alt name
    neurotensin genomic clone
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    8551
  • Mutation
    Added AscI site 3 bp 3' of stop codon
  • Entrez Gene
    Nts (a.k.a. 5033428E16Rik, NMN-125, NN, NT, NT/N, NTS1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (destroyed during cloning)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is the targeting vector for the mouse neurotensin gene. It has an AscI site 3 bp downstream of the stop codon for cassette insertion.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-NT-AscI was a gift from Peter Mombaerts (Addgene plasmid # 15461 ; http://n2t.net/addgene:15461 ; RRID:Addgene_15461)
  • For your References section:

    Development and topography of the lateral olfactory tract in the mouse: imaging by genetically encoded and injected fluorescent markers. Walz A, Omura M, Mombaerts P. J Neurobiol. 2006 Jul . 66(8):835-46. 10.1002/neu.20266 PubMed 16673392