pBS-NT-AscI
(Plasmid
#15461)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 15461 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepBluescript II SK(-)
-
Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 2900
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameneurotensin
-
Alt nameneurotensin genomic clone
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)8551
-
MutationAdded AscI site 3 bp 3' of stop codon
-
Entrez GeneNts (a.k.a. 5033428E16Rik, NMN-125, NN, NT, NT/N, NTS1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (destroyed during cloning)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This is the targeting vector for the mouse neurotensin gene. It has an AscI site 3 bp downstream of the stop codon for cassette insertion.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBS-NT-AscI was a gift from Peter Mombaerts (Addgene plasmid # 15461 ; http://n2t.net/addgene:15461 ; RRID:Addgene_15461) -
For your References section:
Development and topography of the lateral olfactory tract in the mouse: imaging by genetically encoded and injected fluorescent markers. Walz A, Omura M, Mombaerts P. J Neurobiol. 2006 Jul . 66(8):835-46. 10.1002/neu.20266 PubMed 16673392