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Addgene

pLL2787
(Plasmid #15460)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15460 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCL15
  • Backbone manufacturer
    lab construction
  • Backbone size w/o insert (bp) 6843
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    chloramphenicol/ S. aureus
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    phage phi11 int gene
  • Alt name
    formerly called pTL2787
  • Species
    S. aureus
  • Insert Size (bp)
    1085

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLL2787 was a gift from Chia Lee (Addgene plasmid # 15460 ; http://n2t.net/addgene:15460 ; RRID:Addgene_15460)

  • For your References section:

    Improved single-copy integration vectors for Staphylococcus aureus. Luong TT, Lee CY. J Microbiol Methods. 2007 Jul . 70(1):186-90. 10.1016/j.mimet.2007.04.007 PubMed 17512993
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