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Addgene

pYL112-19
(Plasmid #15459)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15459 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLI50
  • Backbone manufacturer
    lab construct
  • Backbone size w/o insert (bp) 5505
  • Vector type
    S.aureus-E. coli shuttle vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    chloramphenicol/ S. aureus
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    S. aureus phage L54a integrase
  • Species
    S. aureus phage L54a int gene
  • Insert Size (bp)
    1600

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PvuII (destroyed during cloning)
  • 3′ cloning site PvuII (destroyed during cloning)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYL112-19 was a gift from Chia Lee (Addgene plasmid # 15459 ; http://n2t.net/addgene:15459 ; RRID:Addgene_15459)

  • For your References section:

    Improved single-copy integration vectors for Staphylococcus aureus. Luong TT, Lee CY. J Microbiol Methods. 2007 Jul . 70(1):186-90. 10.1016/j.mimet.2007.04.007 PubMed 17512993
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