pLL39
(Plasmid #15458)

• Depositing Lab: Chia Lee
• Publication: Luong et al J Microbiol Methods. 2007 Jul . 70(1):186-90.

Backbone

• Vector backbone: N/A
• Backbone size w/o insert (bp): 8019
• Vector type: S. aureus-E. coli shuttle vector

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: tetracycline/ S. aureus
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: None
• Insert Size (bp): 8019
• GenBank ID: EF540896

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pCL25 can integrate into the L54a attB site, whereas pLL29 and pLL39 can integrate at either one of the two sites: L54a attB and phi-11 attB. In addition, pLL39 has a tandem terminator at one side
of the multiple cloning sites to prevent read through of the cloned gene from
an external promoter once integrated into the chromosome.