pLL29
(Plasmid
#15457)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 15457 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneN/A
- Backbone size w/o insert (bp) 7029
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Vector typeS. aureus-E. coli shuttle vector
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionstetracycline/ S. aureus
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
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Insert Size (bp)7029
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GenBank IDEF540895
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pCL25 can integrate into the L54a attB site, whereas pLL29 and pLL39 can integrate at either one of the two sites: L54a attB and phi-11 attB.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLL29 was a gift from Chia Lee (Addgene plasmid # 15457 ; http://n2t.net/addgene:15457 ; RRID:Addgene_15457) -
For your References section:
Improved single-copy integration vectors for Staphylococcus aureus. Luong TT, Lee CY. J Microbiol Methods. 2007 Jul . 70(1):186-90. 10.1016/j.mimet.2007.04.007 PubMed 17512993