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Addgene

human TF cDNA
(Plasmid #15455)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15455 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC8
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 2665
  • Vector type
    Bacterial Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tissue factor
  • Alt name
    TF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    641
  • Entrez Gene
    F3 (a.k.a. CD142, TF, TFA)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13R
  • 3′ sequencing primer M13-F20
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mackman lab pCTF 314.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    human TF cDNA was a gift from Nigel Mackman (Addgene plasmid # 15455 ; http://n2t.net/addgene:15455 ; RRID:Addgene_15455)

  • For your References section:

    Regulation of the tissue factor gene. Mackman N. FASEB J. 1995 Jul . 9(10):883-9. PubMed 7615158
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