mouse TF cDNA
(Plasmid
#15454)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 15454 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepGEM-3Z
-
Backbone manufacturerPromega
- Backbone size w/o insert (bp) 2743
-
Vector typeBacterial Expression, Luciferase
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameTissue factor
-
Alt nameTF
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)818
-
Entrez GeneF3 (a.k.a. CD142, Cf-3, Cf3, TF)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Hinc II (not destroyed)
- 3′ cloning site Hinc II (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Mackman lab #2281.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
mouse TF cDNA was a gift from Nigel Mackman (Addgene plasmid # 15454 ; http://n2t.net/addgene:15454 ; RRID:Addgene_15454) -
For your References section:
Regulation of the tissue factor gene. Mackman N. FASEB J. 1995 Jul . 9(10):883-9. PubMed 7615158