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Addgene

-33 hTF
(Plasmid #15453)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15453 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL2 Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5600
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tissue factor promoter
  • Alt name
    TF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    47
  • Entrez Gene
    F3 (a.k.a. CD142, TF, TFA)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer na
  • 3′ sequencing primer LucNrev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

-33 to +14 hTF promoter. hTF promoter deletion series, Mackman lab #2539.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    -33 hTF was a gift from Nigel Mackman (Addgene plasmid # 15453 ; http://n2t.net/addgene:15453 ; RRID:Addgene_15453)

  • For your References section:

    Regulation of the tissue factor gene. Mackman N. FASEB J. 1995 Jul . 9(10):883-9. PubMed 7615158
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