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Addgene

-111 hTF m3
(Plasmid #15450)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15450 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL2 Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5600
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tissue factor promoter
  • Alt name
    TF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    125
  • Mutation
    All 3 EGR-1 sites mutated
  • Entrez Gene
    F3 (a.k.a. CD142, TF, TFA)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer na
  • 3′ sequencing primer LucNrev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

-111 to +14 hTF promoter with all 3 EGR-1 sites mutated. hTF promoter deletion/mutation series 2, Mackman lab #2447.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    -111 hTF m3 was a gift from Nigel Mackman (Addgene plasmid # 15450 ; http://n2t.net/addgene:15450 ; RRID:Addgene_15450)

  • For your References section:

    Transcriptional regulation of the tissue factor gene in human epithelial cells is mediated by Sp1 and EGR-1. Cui MZ, Parry GC, Oeth P, Larson H, Smith M, Huang RP, Adamson ED, Mackman N. J Biol Chem. 1996 Feb 2. 271(5):2731-9. 10.1074/jbc.271.5.2731 PubMed 8576248
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